Stability of Erythrocytes Fixed in Osmium Tetroxide Solutions

Microtrabecular lattice of the cytoplasmic ground substance. Artifact or reality

The cytoplasmic ground substance of cultured cells prepared for high voltage transmission electron microscopy (glutaraldehyde/osmium fixed, alcohol or acetone dehydrated, critical-point dried) consists of slender (3-6 nm Diam) strands--the microtrabeculae (55)--that form an irregular three-dimensional lattice (the microtrabecular lattice). The microtrabeculae interconnect the membranous and non...

Differences in Membrane Configuration between Osmium Tetroxide-fixed and Glutaraldehyde-fixed Ciliary Epithelium

Preservation of the Lipids of the Human Erythrocyte Stroma during Fixation and Dehydration for Electron Microscopy

Recent reports of lipid and/or protein losses from biological material during fixation processes (Luft and Wood, 1963; Ashworth et al., 1966; Korn and Weisman, 1966; Morgan and Huber, 1967; Stein and Stein, 1967 a, b, 1968; Buschmann and Taylor, 1968; Ongun et al., 1968) emphasize the necessity for basing the interpretation of electron micrographs upon knowledge of the final composition of the ...

Osmium: An Appraisal of Environmental Exposure

In the U.S., the chief source of new osmium is copper refining, where this metal is produced as a byproduct. Probably less than 10% of the osmium in the original copper ore is recovered, and 1000-3000 oz troy of osmium is lost each year to the environment as the toxic, volatile tetroxide from copper smelters. In 1971, about 2000 oz troy of osmium was domestically refined, most of which was from...

The Demonstration of Glycogen in Liver Cells fixed in Osmium Tetroxide

Glycogen can be demonstrated histochemically in mammalian liver cells fixed in osmium tetroxide and embedded either in gelatine or in media used in electron microscopy. The form and distribution of the glycogen correspond very closely with those of structures seen in electron micrographs and which have been presumed to be glycogen. Evidence was obtained which suggests that the glycogen occurrin...